The invertebrate Caenorhabditis elegans biosynthesizes ascorbate.

l-Ascorbate, commonly known as vitamin C, serves as an antioxidant and cofactor essential for many biological processes. Distinct ascorbate biosynthetic pathways have been established for animals and plants, but little is known about the presence or synthesis of this molecule in invertebrate species. We have investigated ascorbate metabolism in the nematode Caenorhabditis elegans, where this molecule would be expected to play roles in oxidative stress resistance and as cofactor in collagen and neurotransmitter synthesis. Using high-performance liquid chromatography and gas-chromatography mass spectrometry, we determined that ascorbate is present at low amounts in the egg stage, L1 larvae, and mixed animal populations, with the egg stage containing the highest concentrations. Incubating C. elegans with precursor molecules necessary for ascorbate synthesis in plants and animals did not significantly alter ascorbate levels. Furthermore, bioinformatic analyses did not support the presence in C. elegans of either the plant or the animal biosynthetic pathway. However, we observed the complete (13)C-labeling of ascorbate when C. elegans was grown with (13)C-labeled Escherichia coli as a food source. These results support the hypothesis that ascorbate biosynthesis in invertebrates may proceed by a novel pathway and lay the foundation for a broader understanding of its biological role

Uncinaria hamiltoni (Nematoda: Ancylostomatidae) in South American Sea Lions, Otaria flavescens, From Northern Patagonia, Argentina

Thirty-one South American sea lion pups (Otaria flavescens) found dead in Punta León, Argentina, during the summer of 2002, were examined for hookworms (Uncinaria hamiltoni). Parasite parameters were analyzed in 2 locations of the rookery, i.e., a traditional, well-structured breeding area and an expanding area with juveniles and a lax social structure. Prevalence of hookworms was 50% in both localities, and no difference was observed in prevalence between pup sexes (P > 0.05). Hookworms were concentrated in the small intestine. Transmammary transmission is assumed because only adult hookworms were found in the pups. The mean intensity of hookworms per pup was 135; the mean intensity in females (92.78) was significantly different (P 0.05) in intensity was found between the 2 breeding areas, although prevalence was higher in the traditional breeding area than in the other area. Location was the only factor affecting hookworm prevalence (P log-linear model: 0.9552; χ2: 1.5629). No apparent trend between body condition and intensity of hookworms was observed.Raga Esteve, Juan Antonio, [email protected]

Gnathostomosis, an emerging foodborne zoonotic disease in Acapulco, Mexico.

Between 1993 and 1997, 98 gnathostomosis cases were clinically identified in Acapulco, Mexico. Intermittent cutaneous migratory swellings were the commonest manifestation. Larvae were identified in 26 cases, while in 72, final diagnosis was made on the basis of epidemiologic data, food habits, and positive enzyme-linked immunosorbent assay and Western blot results

Numancia : novela histórica

Copia digital. Valladolid : Junta de Castilla y León. Consejería de Cultura y Turismo, 2009-201

Casimir force in brane worlds: coinciding results from Green's and Zeta function approaches

Casimir force encodes the structure of the field modes as vacuum fluctuations and so it is sensitive to the extra dimensions of brane worlds. Now, in flat spacetimes of arbitrary dimension the two standard approaches to the Casimir force, Green's function and zeta function, yield the same result, but for brane world models this was only assumed. In this work we show both approaches yield the same Casimir force in the case of Universal Extra Dimensions and Randall-Sundrum scenarios with one and two branes added by p compact dimensions. Essentially, the details of the mode eigenfunctions that enter the Casimir force in the Green's function approach get removed due to their orthogonality relations with a measure involving the right hyper-volume of the plates and this leaves just the contribution coming from the Zeta function approach. The present analysis corrects previous results showing a difference between the two approaches for the single brane Randall-Sundrum; this was due to an erroneous hyper-volume of the plates introduced by the authors when using the Green's function. For all the models we discuss here, the resulting Casimir force can be neatly expressed in terms of two four dimensional Casimir force contributions: one for the massless mode and the other for a tower of massive modes associated with the extra dimensions.Comment: 30 pages, title, abstract and discussion have change

Identificaci?n y propuesta de recuperaci?n del edificio antigua sede colegio I.T.A.C. en el corregimiento de Villa Restrepo ? Ibagu?

150 p. Recurso Electr?nicoEl proyecto consiste en realizar la identificaci?n, reconocimiento y propuesta de intervenci?n del edificio de la antigua sede del colegio I.T.A.C. (Instituto T?cnico Ambiental Combeima) ubicado en el corregimiento de Villa Restrepo, zona del ca??n del Combeima en la ciudad de Ibagu?; con el prop?sito de rescatar el edificio, tambi?n de aportar a la comunidad la satisfacci?n de una necesidad respecto al uso que se le otorgue a este, y tambi?n, devolverle la memoria colectiva del lugar y el sentido de pertenencia que mediante la propuesta va a convertir al edificio en un lugar para todos. La intervenci?n busca mejorar los espacios existentes en el sector, articular los bienes de inter?s cultural en un proyecto de renovaci?n urbana con el centro del corregimiento, inmuebles perif?ricos y el componente ambiental que busca de organizar y rentabilidad a trav?s de sus usos, rescatando los valores de inter?s patrimonial y cultural. Palabras Clave: Patrimonio, Intervenci?n, Cultura Campesina, Rural, Colegio, Centro Cultural.The project consists of carrying out the identification, recognition and intervention proposal of the building of the former headquarters of the school I.T.A.C. (Combeima Technical Environmental Institute) located in the corregimiento of Villa Restrepo, zone of the Combeima canyon in the city of Ibagu?; with the purpose of rescuing the building, to contribute to the community satisfaction of a need with respect to the use that is granted to it, and also, to return the collective memory of the place and the sense of belonging that through the proposal will convert to the building in a place for everyone. The intervention seeks to improve the existing spaces in the sector, articulate cultural assets in a project of urban renewal with the center of the corregimiento, peripheral buildings and the environmental component that seeks to organize and profitability through their uses, rescuing the values of heritage and cultural interest. Keywords: Heritage, Intervention, Peasant Culture, Rural, School, Cultural Center

Alloy Design for Additive Manufacturing:Early-Stage Oxidation of Nickel-Based Superalloys

This body of work aims to inform alloy design for additive manufacturing by investigating the early-stage oxidation behavior of Ni-based superalloys processed by laser-powder bed fusion. The oxidation of 14 Ni-based superalloys—some novel and some heritage—at 1000 °C for 24 hours is studied through thermo-gravimetric analysis. The mass gain, oxide layer thickness, oxide scale composition, and depletion γ' zone size are measured. The influence of the alloy composition on these variables is assessed in order to elucidate how increasingly processable and oxidation resistant alloys can be developed. The alloy compositions with Al content greater than 9 at. pct form continuous Al2O3 scales at 1000 °C and display markedly lower parabolic rate constants, mass gain, oxide layer thickness, and γ' depletion zone size. The alloys of lesser Al content have reduced oxidation resistance and formed oxide scales of predominantly Cr2O3. Alloys with Ti content of 2.7 at. pct and greater formed Ti-rich oxide phases in their oxide scales as well as TiN subscale. A trade-off between alloy processability and oxidation resistance is identified, dictated by the deleterious effect of Al content on the ductility dip and the benefit of Al for oxidation resistance. A property space along the pareto front is highlighted which is ideal for having oxidation resistance and processability

Garlic's ability to prevent in vitro Cu(2+)-induced lipoprotein oxidation in human serum is preserved in heated garlic: effect unrelated to Cu(2+)-chelation

BACKGROUND: It has been shown that several extracts and compounds derived from garlic are able to inhibit Cu(2+)-induced low density lipoprotein oxidation. In this work we explored if the ability of aqueous garlic extract to prevent in vitro Cu(2+)-induced lipoprotein oxidation in human serum is affected by heating (a) aqueous garlic extracts or (b) garlic cloves. In the first case, aqueous extract of raw garlic and garlic powder were studied. In the second case, aqueous extract of boiled garlic cloves, microwave-treated garlic cloves, and pickled garlic were studied. It was also studied if the above mentioned preparations were able to chelate Cu(2+). METHODS: Cu(2+)-induced lipoprotein oxidation in human serum was followed by the formation of conjugated dienes at 234 nm and 37°C by 240 min in a phosphate buffer 20 mM, pH 7.4. Blood serum and CuSO(4 )were added to a final concentration of 0.67% and 0.0125 mM, respectively. The lag time and the area under the curve from the oxidation curves were obtained. The Cu(2+)-chelating properties of garlic extracts were assessed using an approach based upon restoring the activity of xanthine oxidase inhibited in the presence of 0.050 mM Cu(2+). The activity of xanthine oxidase was assessed by monitoring the production of superoxide anion at 560 nm and the formation of uric acid at 295 nm. Data were compared by parametric or non-parametric analysis of variance followed by a post hoc test. RESULTS: Extracts from garlic powder and raw garlic inhibited in a dose-dependent way Cu(2+)-induced lipoprotein oxidation. The heating of garlic extracts or garlic cloves was unable to alter significantly the increase in lag time and the decrease in the area under the curve observed with the unheated garlic extracts or raw garlic. In addition, it was found that the garlic extracts were unable to chelate Cu(2+). CONCLUSIONS: (a) the heating of aqueous extracts of raw garlic or garlic powder or the heating of garlic cloves by boiling, microwave or pickling do not affect garlic's ability to inhibit Cu(2+)-induced lipoprotein oxidation in human serum, and (b) this ability is not secondary to Cu(2+)-chelation